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Cell carrier function of hollow-fiber membrane in rotating wall vessel bioreactor

SONG Kedong, LIU Tianqing, ZHAO Hu, LI Xiangqin, MA Xuehu, CUI Zhanfeng

《化学科学与工程前沿（英文）》 2008年 第2卷 第1期   页码 34-39 doi: 10.1007/s11705-008-0022-3

摘要： Large-scale expansion of the osteoblasts of a Sprague-Dawley (SD) rat was studied in a rotating wall hollow-fiber membrane bioreactor (RWHMB) by using hollow-fiber membrane as the carrier. For the sake of contrast, cells were also expanded in a T-flask using a hollow-fiber membrane as carrier and in a rotating wall vessel bioreactor (RWVB) using a microcarrier. During the culture period, the cells were sampled every 12 h, and after 5 days, the cells were harvested and evaluated with scanning electron microscopy (SEM), hematoxylin-eosin (HE) staining and alkaline phosphatase (ALP) staining. Moreover, von-Kossa staining and Alizarin Red S staining were carried out for mineralized nodules formation. The results show that in RWHMB, the cells present better morphology and vitality and secrete much more extracellular matrix. It is concluded that the RWHMB combines the advantages of the rotating wall vessel and hollow-fiber membrane bioreactors. The hydrodynamic stimulation within it accelerates the metabolism of the osteoblast and mass transfer, which is propitious to cell differentiation and proliferation.

关键词： RWHMB     microcarrier     hematoxylin-eosin     von-Kossa staining     propitious    

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Epidemiological study of a von Hippel-Lindau family in northwest China

null

《医学前沿（英文）》 2013年 第7卷 第3期   页码 378-385 doi: 10.1007/s11684-013-0276-0

摘要：

von Hippel-Lindau (VHL) disease is a rare, inherited neoplastic disease characterized by hemangioblastomas (HBL) of the central nervous system (CNS), retinal angiomas, renal cell carcinomas (RCC), pancreatic endocrine tumors (PETs), pheochromocytomas, paragangliomas, and visceral cysts. We encountered a large VHL family in northwest China and conducted a systematic screening of the family members based on their epidemiological and clinical characteristics. A self-designed questionnaire was used to collect the general sociodemographic and health information of the family members. For the preliminary family screening, physical examination and abdomen B ultrasonography were performed. The suspected patients were subjected to cranial computerized tomography and fundus examination. The clinical data of the patients with confirmed VHL disease were collected from hospital records. A total of 63 lineal descendants in six generations were observed in the family (generations O, A, B, C, D, E), including 9 dead suspected cases (6 males, 3 females) and 10 living cases (2 males, 8 females). Among the 10 living cases, 4, 2, 1, 3, 4, 8, and 2 manifested HBLs of the CNS, PETs, RCC, pancreatic cysts, renal cysts, pheochromocytomas (4 hemi and 4 bilateral), and paragangliomas, respectively. Data showed that the morbidity of VHL disease in generation C was lower than that in generation B, but the age of onset was younger. This study is the first to report VHL disease in northwest China and VHL-associated PET cases in Chinese. Therefore, follow-up checkups of the family should be focused on younger generations. Proper family screening protocols should be followed for the treatment of patients with VHL disease.

关键词： von Hippel-Lindau disease     epidemiology     family screening     pancreatic endocrine tumor    

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What have we known so far for fluorescence staining and quantification of microplastics: A tutorial review

Shengdong Liu, Enxiang Shang , Jingnan Liu , Yining Wang , Nanthi Bolan , M.B. Kirkham , Yang Li

《环境科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 8-8 doi: 10.1007/s11783-021-1442-2

摘要： Understanding the fate and toxicity of microplastics (MPs,<5 mm plastic particles) is limited by quantification methods. This paper summarizes the methods in use and presents new ones. First, sampling and pretreatment processes of MPs, including sample collection, digestion, density separation, and quality control are reviewed. Then the promising and convenient staining procedures and quantification methods for MPs using fluorescence dyes are reviewed. The factors that influence the staining of MPs, including their physicochemical properties, are summarized to provide an optimal operation procedure. In general, the digestion step is crucial to eliminate natural organic matter (NOM) to avoid interference in quantification. Chloroform was reported to be the most appropriate solvent, and 10–20 μg/mL are recommended as optimal dye concentrations. In addition, a heating and cooling procedure is recommended to maintain the fluorescence intensity of MPs for two months. After staining, a fluorescence microscope is usually used to characterize the morphology, mass, or number of MPs, but compositional analysis cannot be determined with it. These fluorescence staining methods have been implemented to study MP abundance, transport, and toxicity and have been combined with other chemical characterization techniques, such as Fourier transform infrared spectroscopy and Raman spectroscopy. More studies are needed to focus on the synthesis of novel dyes to avoid NOM’s interference. They need to be combined with other spectroscopic techniques to characterize plastic composition and to develop image-analysis methods. The stability of stained MPs needs to be improved.
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The carbon dioxide removal potential of Liquid Air Energy Storage: A high-level technical and economic appraisal

Andrew LOCKLEY, Ted von HIPPEL

《工程管理前沿（英文）》 2021年 第8卷 第3期   页码 456-464 doi: 10.1007/s42524-020-0102-8

摘要： Liquid Air Energy Storage (LAES) is at pilot scale. Air cooling and liquefaction stores energy; reheating revaporises the air at pressure, powering a turbine or engine (Ameel et al., 2013). Liquefaction requires water & CO removal, preventing ice fouling. This paper proposes subsequent geological storage of this CO – offering a novel Carbon Dioxide Removal (CDR) by-product, for the energy storage industry. It additionally assesses the scale constraint and economic opportunity offered by implementing this CDR approach. Similarly, established Compressed Air Energy Storage (CAES) uses air compression and subsequent expansion. CAES could also add CO scrubbing and subsequent storage, at extra cost. CAES stores fewer joules per kilogram of air than LAES – potentially scrubbing more CO per joule stored. Operational LAES/CAES technologies cannot offer full-scale CDR this century (Stocker et al., 2014), yet they could offer around 4% of projected CO disposals for LAES and<25% for current-technology CAES. LAES CDR could reach trillion-dollar scale this century (20 billion USD/year, to first order). A larger, less certain commercial CDR opportunity exists for modified conventional CAES, due to additional equipment requirements. CDR may be commercially critical for LAES/CAES usage growth, and the necessary infrastructure may influence plant scaling and placement. A suggested design for low-pressure CAES theoretically offers global-scale CDR potential within a century (ignoring siting constraints) – but this must be costed against competing CDR and energy storage technologies.

关键词： carbon dioxide removal     Liquid Air Energy Storage     Compressed Air Energy Storage     geoengineering    

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The epidemiology of norovirus gastroenteritis in China: disease burden and distribution of genotypes

Honglu Zhou, Songmei Wang, Lorenz von Seidlein, Xuanyi Wang

《医学前沿（英文）》 2020年 第14卷 第1期   页码 1-7 doi: 10.1007/s11684-019-0733-5

摘要： With the improvements of sanitation and nationwide safe water supply the occurrence of bacterial diarrhea declined remarkably, while viruses became the leading causes of acute gastroenteritis (AGE). Of these viruses, noroviruses (NoVs) are responsible for a considerable burden of gastroenteritis, especially in children<2 years and elderly≥65 years. NoVs circulating in the Chinese population are antigenically highly diverse with the genotype GII.4 being the dominant strain followed by GII.3. Given the widespread contamination in environmental sources, and highly infectious nature of NoVs, vaccination would be the desirable strategy for the control of NoV infections. However, a better understanding of acquired immunity after infection, and a reliable immunological surrogate marker are urgently needed, since two vaccine candidates based on virus-like particles (VLPs) are currently moving into clinical evaluations in China.

关键词： molecular epidemiology     norovirus     disease burden     genotype     China    

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Transient analysis of thermoelastic contact problem of disk brakes

Ali BELHOCINE, Mostefa BOUCHETARA

《机械工程前沿（英文）》 2013年 第8卷 第2期   页码 150-159 doi: 10.1007/s11465-013-0266-6

摘要：

The main purpose of this study is to analyze the thermomechanical behavior of the dry contact between the brake disk and pads during the braking phase. The simulation strategy is based on computer code ANSYS11. The modeling of transient temperature in the disk is actually used to identify the factor of geometric design of the disk to install the ventilation system in vehicles. The thermal-structural analysis is then used with coupling to determine the deformation established and the Von Mises stresses in the disk, the contact pressure distribution in pads. The results are satisfactory compared to those found in the literature.

关键词： brake disks     heat flux     heat-transfer coefficient     Von Mises stress     contact pressure    

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Extrapolation reconstruction of wind pressure fields on the claddings of high-rise buildings

Yehua SUN, Guquan SONG, Hui LV

《结构与土木工程前沿（英文）》 2019年 第13卷 第3期   页码 653-666 doi: 10.1007/s11709-018-0503-5

摘要： Recent research about reconstruction methods mainly used the interpolation reconstruction of the fluctuating wind pressure field on the surface. However, to investigate wind pressure at the edge of the building, the work presented in this paper focuses on the extrapolation reconstruction of wind pressure fields. Here, we propose an improved proper orthogonal decomposition (POD) and Kriging method with a von Kármán correlation function to resolve this issue. The studies show that it works well for not only interpolation reconstruction but also extrapolation reconstruction. The proposed method does require determination of the Hurst exponent and other parameters analysed from the original data. Hence, the fluctuating wind fields have been characterized by the von Kármán correlation function, as an a priori function. Compared with the cubic spline method and different variogram, preliminary results suggest less time consumption and high efficiency in extrapolation reconstruction at the edge.

关键词： extrapolation reconstruction     proper orthogonal decomposition     Kriging method     von Kármán function     Hurst exponent     rescaled range analysis    

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Adaptive selective ES-FEM limit analysis of cracked plane-strain structures

H. NGUYEN-XUAN,T. RABCZUK

《结构与土木工程前沿（英文）》 2015年 第9卷 第4期   页码 478-490 doi: 10.1007/s11709-015-0317-7

摘要： This paper presents a simple and efficient approach for predicting the plastic limit loads in cracked plane-strain structures. We use two levels of mesh repartitioning for the finite element limit analysis. The master level handles an adaptive primal-mesh process through a dissipation-based indicator. The slave level performs the subdivision of each triangle into three sub-triangles and constitutes a dual mesh from a pair of two adjacent sub-triangles shared by common edges of the primal mesh. Applying a strain smoothing projection to the strain rates on the dual mesh, the incompressibility constraint and the flow rule constraint are imposed over the edge-based smoothing domains and everywhere in the problem domain. The limit analysis problem is recast into the compact form of a second-order cone programming (SOCP) for the purpose of exploiting interior-point solvers. The present method retains a low number of optimization variables. It offers a convenient way for designing and solving the large-scale optimization problems effectively. Several benchmark examples are given to show the simplicity and effectiveness of the present method.

关键词： cracked structure     limit analysis     von Mises criterion     edge-based strain smoothing     second-order cone programming     adaptive    

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Investigating peak stresses in fitting and repair patches of buried polyethylene gas pipes

Reza KHADEMI ZAHEDI, Pouyan ALIMOURI, Hooman KHADEMI ZAHEDI, Mohammad SHISHESAZ

《结构与土木工程前沿（英文）》 2020年 第14卷 第1期   页码 147-168 doi: 10.1007/s11709-019-0587-6

摘要： Nowadays, polyethylene composes a large number of natural gas distribution pipelines installed under the ground. The focus of the present contribution is two fold. One of the objectives is to investigate the applicability of polyethylene fittings in joining polyethylene gas pipes which are electrofused onto the pipe ends and buried under the ground, by estimating stress distribution using finite element method. The second objective is to study the effectiveness of polyethylene repair patches which are used to mend the defected pipelines by performing a finite element analysis to calculate peak stress values. Buried polyethylene pipelines in the natural gas industry, can be imposed by sever loadings including the soil-structure interaction, traffic load, soil’s column weight, internal pressure, and thermal loads resulting from daily and/or seasonal temperature changes. Additionally, due to the application of pipe joints, and repair patches local stresses superimposed on the aforementioned loading effects. The pipe is assumed to be made of PE80 resin and its jointing socket, and the repair patch is PE100 material. The computational analysis of stresses and the computer simulations are performed using ANSYS commercial software. According to the results, the peak stress values take place in the middle of the fitting and at its internal surface. The maximum stress values in fitting and pipe are below the allowable stresses which shows the proper use of introduced fitting is applicable even in hot climate areas of Ahvaz, Iran. Although the buried pipe is imposed to the maximum values of stresses, the PE100 socket is more sensitive to a temperature drop. Furthermore, all four studied patch arrangements show significant reinforcing effects on the defected section of the buried PE gas pipe to transfer applied loads. Meanwhile, the defected buried medium density polyethylene gas pipe and its saddle fused patch can resist the imposed mechanical and thermal loads of 22°C temperature increase. Moreover, increasing the saddle fusion patch length to 12 inches reduces the maximum stress values in the pipe, significantly.

关键词： Ansys software     polyethylene     buried pipelines     polyethylene joints     polyethylene patches     peak von Mises stress     soil-pipe interaction     temperature variation    

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Elasto-plastic fatigue crack growth analysis of plane problems in the presence of flaws using XFEM

Sachin KUMAR,A. S. SHEDBALE,I. V. SINGH,B. K. MISHRA

《结构与土木工程前沿（英文）》 2015年 第9卷 第4期   页码 420-440 doi: 10.1007/s11709-015-0305-y

摘要： In this paper, elasto-plastic XFEM simulations have been performed to evaluate the fatigue life of plane crack problems in the presence of various defects. The stress-strain response of the material is modeled by Ramberg-Osgood equation. The von-Mises failure criterion has been used with isotropic hardening. The -integral for two fracture modes (mode-I and mode-II) is obtained by decomposing the displacement and stress fields into their symmetric and antisymmetric parts, then individual stress intensity factors are extracted from -integral. The fatigue life obtained by EPFM is found quite close to that obtained by LEFM.

关键词： XFEM     von-Mises yield criterion     isotropic hardening     fatigue crack growth     J-integral    

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标题 作者 时间 类型 操作

Cell carrier function of hollow-fiber membrane in rotating wall vessel bioreactor

SONG Kedong, LIU Tianqing, ZHAO Hu, LI Xiangqin, MA Xuehu, CUI Zhanfeng

期刊论文

Epidemiological study of a von Hippel-Lindau family in northwest China

null

期刊论文

What have we known so far for fluorescence staining and quantification of microplastics: A tutorial review

Shengdong Liu, Enxiang Shang , Jingnan Liu , Yining Wang , Nanthi Bolan , M.B. Kirkham , Yang Li

期刊论文

The carbon dioxide removal potential of Liquid Air Energy Storage: A high-level technical and economic appraisal

Andrew LOCKLEY, Ted von HIPPEL

期刊论文

The epidemiology of norovirus gastroenteritis in China: disease burden and distribution of genotypes

Honglu Zhou, Songmei Wang, Lorenz von Seidlein, Xuanyi Wang

期刊论文

Transient analysis of thermoelastic contact problem of disk brakes

Ali BELHOCINE, Mostefa BOUCHETARA

期刊论文

Extrapolation reconstruction of wind pressure fields on the claddings of high-rise buildings

Yehua SUN, Guquan SONG, Hui LV

期刊论文

Adaptive selective ES-FEM limit analysis of cracked plane-strain structures

H. NGUYEN-XUAN,T. RABCZUK

期刊论文

Investigating peak stresses in fitting and repair patches of buried polyethylene gas pipes

Reza KHADEMI ZAHEDI, Pouyan ALIMOURI, Hooman KHADEMI ZAHEDI, Mohammad SHISHESAZ

期刊论文

Elasto-plastic fatigue crack growth analysis of plane problems in the presence of flaws using XFEM

Sachin KUMAR,A. S. SHEDBALE,I. V. SINGH,B. K. MISHRA

期刊论文